Research Paper Volume 13, Issue 15 pp 19643—19656

The protective effect of allicin on myocardial ischemia-reperfusion by inhibition of Ca2+ overload-induced cardiomyocyte apoptosis via the PI3K/GRK2/PLC-γ/IP3R signaling pathway

Microarray analysis exposed the regulation of the calcium ion pathway, and gene PI3KCA (PI3K) might influence the allicin-induced MI/R protection. (A) The hierarchical clustering analysis exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (B) The volcano plot exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (C) The qPCR analysis revealed that the level of PI3KCA(PI3K) was significantly decreased in MI/R+ Allicin tissues compared with I/R tissues. (P D) The receiver operating characteristic (ROC) curve revealed that the expression level of PI3KCA is associated with a higher overall survival rate. (E) The annotations of Gene Ontology (GO) found 39 upregulated pathways. (F) The annotations of Gene Ontology (GO) found 37 downregulated pathways (Figure 3E). (G) The partial results of the KEGG pathways.

Figure 3. Microarray analysis exposed the regulation of the calcium ion pathway, and gene PI3KCA (PI3K) might influence the allicin-induced MI/R protection. (A) The hierarchical clustering analysis exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (B) The volcano plot exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (C) The qPCR analysis revealed that the level of PI3KCA(PI3K) was significantly decreased in MI/R+ Allicin tissues compared with I/R tissues. (P < 0.05, MI/R vs. Allicin). (D) The receiver operating characteristic (ROC) curve revealed that the expression level of PI3KCA is associated with a higher overall survival rate. (E) The annotations of Gene Ontology (GO) found 39 upregulated pathways. (F) The annotations of Gene Ontology (GO) found 37 downregulated pathways (Figure 3E). (G) The partial results of the KEGG pathways.