Research Paper Volume 13, Issue 16 pp 20192—20217

Hsa-circ-0007292 promotes the osteogenic differentiation of posterior longitudinal ligament cells via regulating SATB2 by sponging miR-508-3p

Hsa

Figure 4. Hsa_circ_0007292 serves as a sponge for miR-508-3p. (A) Schematic exhibiting the overlapping target miRNAs of hsa_circ_0007292 predicted by the circInteractome, StarBase, and circBank databases. (B) The relative expression of the four overlapping miRNAs was investigated by qRT-PCR assay in OPLL cells (n=6) and non-OPLL cells (n=6). (C) The correlation between relative miR-508-3p expression and relative hsa_circ_0007292 expression in OPLL cells (n=8, R2=-0.5788, P=0.0284). (D) Schematic of the predicted target site between miR-508-3p and hsa_circ_0007292 from circInteractome and StarBase online databases. (E) The activity of hsa_circ_0007292 dual-luciferase reporter was detected in HEK-293T cells cotransfected with miR-508-3p mimic or mimic control(n=3). (F) Hsa_circ_0007292 and miR-508-3p simultaneously showed a higher expression in the anti-Ago2 precipitation product(n=3). All experiments were performed at least three times. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P<0.001.