Figure 5. MiR-508-3p suppresses the osteogenic differentiation of PLL cells. (A) The level of miR-508-3p was significantly suppressed after treatment with the miR-508-3p inhibitor compared to treatment with the negative control(n=3). (B) The ALP staining showed that the osteogenic properties of non-OPLL cells were enhanced after treated with miR-508-3p inhibitor. (C, D) An increased expression of osteogenic differentiation-related markers was detected by qRT-PCR and Western Blot(n=3). (E) MiR-508-3p was successfully overexpressed after treated OPLL cells with the miR-508-3p mimic(n=3). (F) The ALP activity of OPLL cells was suppressed by miR-508-3p mimic. (G, H) The expression of osteogenic differentiation-related markers was suppressed at the mRNA and protein levels after transfection with the miR-508-3p mimic versus the negative control(n=3). All tests were conducted at least three times. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P<0.001.