Research Paper Volume 13, Issue 15 pp 19127—19144

The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging process

Age dependent expression of SPRR2C and conservation in evolution. In (A) the ΔΔCt values of SPPR2C in foreskin samples of middle aged (age range: 24-39 years) and elderly people (age range: 60 to 76 years) are shown after normalization to young foreskin samples (age range: 2.5-8 years), (N=5 in each group;). One-way ANOVA analysis indicates a significant difference between groups (p= 0.0153285). In (B) a multiple sequence alignment (first 54 nucleotides of the ORF) of SPRR2C in hominids, apes and mammals was calculated using MVIEW (<a href="https://www.ebi.ac.uk/Tools/msa/mview/" target="

Figure 1. Age dependent expression of SPRR2C and conservation in evolution. In (A) the ΔΔCt values of SPPR2C in foreskin samples of middle aged (age range: 24-39 years) and elderly people (age range: 60 to 76 years) are shown after normalization to young foreskin samples (age range: 2.5-8 years), (N=5 in each group;). One-way ANOVA analysis indicates a significant difference between groups (p= 0.0153285). In (B) a multiple sequence alignment (first 54 nucleotides of the ORF) of SPRR2C in hominids, apes and mammals was calculated using MVIEW (https://www.ebi.ac.uk/Tools/msa/mview/). In most hominids the 6th codon (CAG) encodes a glutamine but is changed into a STOP codon (TAG) in Homo sapiens, Pan troglodytes and Pan paniscus. 1: Homo sapiens; 2: Pan troglodytes; 3: Pan paniscus; 4: Gorilla gorilla gorilla; 5: Pongo abelii; 6: Sapajus paella; 7: Papio Anubis; 8: Cebus capucinus; 9: Chlorocebus sabaeus; and 10: Condylura cristata.