Figure 1. Age dependent expression of SPRR2C and conservation in evolution. In (A) the ΔΔCt values of SPPR2C in foreskin samples of middle aged (age range: 24-39 years) and elderly people (age range: 60 to 76 years) are shown after normalization to young foreskin samples (age range: 2.5-8 years), (N=5 in each group;). One-way ANOVA analysis indicates a significant difference between groups (p= 0.0153285). In (B) a multiple sequence alignment (first 54 nucleotides of the ORF) of SPRR2C in hominids, apes and mammals was calculated using MVIEW (https://www.ebi.ac.uk/Tools/msa/mview/). In most hominids the 6th codon (CAG) encodes a glutamine but is changed into a STOP codon (TAG) in Homo sapiens, Pan troglodytes and Pan paniscus. 1: Homo sapiens; 2: Pan troglodytes; 3: Pan paniscus; 4: Gorilla gorilla gorilla; 5: Pongo abelii; 6: Sapajus paella; 7: Papio Anubis; 8: Cebus capucinus; 9: Chlorocebus sabaeus; and 10: Condylura cristata.