Research Paper Volume 13, Issue 15 pp 19127—19144

The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging process

The role of the lncRNA SPRR2c and hsa-mir542-5p in keratinocyte differentiation. RT-PCR analysis of selected CE family members are presented as ΔΔCt values in (A, B). In (A) SPRR2C (pLL3.7-SPRR2c) is overexpressed after transduction of HaCaT cells with lentiviral particles. In (B) the increase of expression of several CE family members is analyzed after addition of 0.8 mM CaCl2 and transfection of HaCaT cells with hsa-mir542a-5p (N=4; *: p

Figure 5. The role of the lncRNA SPRR2c and hsa-mir542-5p in keratinocyte differentiation. RT-PCR analysis of selected CE family members are presented as ΔΔCt values in (A, B). In (A) SPRR2C (pLL3.7-SPRR2c) is overexpressed after transduction of HaCaT cells with lentiviral particles. In (B) the increase of expression of several CE family members is analyzed after addition of 0.8 mM CaCl2 and transfection of HaCaT cells with hsa-mir542a-5p (N=4; *: p<0.1; **: p<0.05; ***: p<0.01).