Research Paper Volume 13, Issue 15 pp 19127—19144

The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging process

The doubling time of HaCaT cells in dependence of calcium and mir542-5p. HaCaT cells were treated with 0.5, 0.8 and 1 mM CaCl2 to induce the formation of the cornified envelope. In parallel cells were transfected with a control miRNA and hsa-mir542-5p. Cell numbers were calculated 24, 48 and 72 hours after start of treatment (N=4-6; *: p

Figure 6. The doubling time of HaCaT cells in dependence of calcium and mir542-5p. HaCaT cells were treated with 0.5, 0.8 and 1 mM CaCl2 to induce the formation of the cornified envelope. In parallel cells were transfected with a control miRNA and hsa-mir542-5p. Cell numbers were calculated 24, 48 and 72 hours after start of treatment (N=4-6; *: p<0.1; **: p<0.05; ***: p<0.01).