Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

miR-221-3p targeted SOCS3. (A) The underlying target of miR-221-3p was searched in the Starbase. (B) The target link between miR-221-3p and SOCS3 in Saos2 cells was evaluated by the dual-luciferase reporter assay. (C–D). miR-221-3p mimics, inhibitors or their negative controls were transfected into Saos2 cells. The SOCS3/JAK2/STAT3 expression in Saos2 cells was evaluated by WB. NS P > 0.05, *P **P N = 3.

Figure 5. miR-221-3p targeted SOCS3. (A) The underlying target of miR-221-3p was searched in the Starbase. (B) The target link between miR-221-3p and SOCS3 in Saos2 cells was evaluated by the dual-luciferase reporter assay. (CD). miR-221-3p mimics, inhibitors or their negative controls were transfected into Saos2 cells. The SOCS3/JAK2/STAT3 expression in Saos2 cells was evaluated by WB. NS P > 0.05, *P < 0.05, **P < 0.01 (vs. miR-NC or NC-in group), N = 3.