Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

miR-221-3p overexpression curbed SOCS3 and boosted JAK2/STAT3. miR-221-3p mimics or inhibitors were transferred into M2-TAMs. The exosomes in the culture medium of M2-TAMs were isolated and then treated with Saos2 cells. (A–B) WB was used for analyzing the SOCS3/JAK2/STAT3 pathway expression. **P ***P &&P &&&P TAMs+miR-NC group or EXOTAMs+NC-in group), N = 3.

Figure 6. miR-221-3p overexpression curbed SOCS3 and boosted JAK2/STAT3. miR-221-3p mimics or inhibitors were transferred into M2-TAMs. The exosomes in the culture medium of M2-TAMs were isolated and then treated with Saos2 cells. (AB) WB was used for analyzing the SOCS3/JAK2/STAT3 pathway expression. **P < 0.01, ***P < 0.001 (vs. con group). &&P < 0.01, &&&P < 0.001 (vs. EXOTAMs+miR-NC group or EXOTAMs+NC-in group), N = 3.