miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

Wei Liu; Qiuping Long; Wei Zhang; Dehui Zeng; Bingbing Hu; Shengyao Liu; Li Chen

doi:doi:10.18632/aging.203388

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Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

Figure 6. miR-221-3p overexpression curbed SOCS3 and boosted JAK2/STAT3. miR-221-3p mimics or inhibitors were transferred into M2-TAMs. The exosomes in the culture medium of M2-TAMs were isolated and then treated with Saos2 cells. (A–B) WB was used for analyzing the SOCS3/JAK2/STAT3 pathway expression. ^**P < 0.01, ^***P < 0.001 (vs. con group). ^&&P < 0.01, ^&&&P < 0.001 (vs. EXO^TAMs+miR-NC group or EXO^TAMs+NC-in group), N = 3.