Research Paper Volume 13, Issue 15 pp 19760—19775

miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis

Inhibiting JAK2/STAT3 reversed the oncogenic property of miR-221-3p. LY2784544 (3 nM) was applied to treat Saos2 cells, which were transfected with miR-221-3p mimics. (A) CCK-8 tested cell viability. (B) Cell proliferation was monitored by the colony formation assay. (C) FCM was utilized to detect cell apoptosis. (D) The profiles of Bax, Bcl2 and Cleaved Caspase-3 were assessed by WB. (E) WB was conducted to detect the SOCS3/JAK2/STAT3 profile and its phosphorylation. NSP>0.05, **P ***P &P &&P &&&P N = 3.

Figure 7. Inhibiting JAK2/STAT3 reversed the oncogenic property of miR-221-3p. LY2784544 (3 nM) was applied to treat Saos2 cells, which were transfected with miR-221-3p mimics. (A) CCK-8 tested cell viability. (B) Cell proliferation was monitored by the colony formation assay. (C) FCM was utilized to detect cell apoptosis. (D) The profiles of Bax, Bcl2 and Cleaved Caspase-3 were assessed by WB. (E) WB was conducted to detect the SOCS3/JAK2/STAT3 profile and its phosphorylation. NSP>0.05, **P < 0.01, ***P < 0.001 (vs. con group), &P < 0.05, &&P < 0.01, &&&P < 0.001 (vs. LY2784544 group), N = 3.