Research Paper Volume 13, Issue 15 pp 19805—19821

MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression

MiRNA expression of DF-EVs. (A) Differential expression levels of miRNAs between Control-EVs and DF-EVs were presented in a heatmap. (B) MiRNA network predicted to target VEGFA using FunRich software. (C) Expression levels of candidate miRNAs in DF-EVs were quantitated by qRT-PCR. (D) Candidate miRNAs were detected in sera of nondiabetic patients and diabetic patients with or without foot ulcers. *P **P ***P

Figure 3. MiRNA expression of DF-EVs. (A) Differential expression levels of miRNAs between Control-EVs and DF-EVs were presented in a heatmap. (B) MiRNA network predicted to target VEGFA using FunRich software. (C) Expression levels of candidate miRNAs in DF-EVs were quantitated by qRT-PCR. (D) Candidate miRNAs were detected in sera of nondiabetic patients and diabetic patients with or without foot ulcers. *P < 0.05, **P < 0.01, ***P < 0.001. At least three replicates of each experiment were performed.