Figure 3. CircZNF609 inhibits DNA damage in melanoma cells. (A–D) The A375 and SK-MEL-28 cells were treated with the circZNF609 shRNA or control shRNA. (A and B) The DNA damage was analyzed by comet assays in the cells. (C and D) The protein expression of H2AX, γH2AX and β-actin was determined by Western blot analysis in the cells. The results of Western blot analysis were quantified by ImageJ software. Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01.