Research Paper Volume 13, Issue 17 pp 21191—21201

LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p

PITPNA-AS1 acts as a sponge for miR-92a-3p in pancreatic carcinoma cells. (A) Subcellular fractionation assay was performed to identify the location of PITPNA-AS1 in SGC7901 cells. (B) The binding sites between PITPNA-AS1 and miR-92a-3p; Luciferase activity of miR-92a-3p mimic with PITPNA-AS1-WT or PITPNA-AS1-MUT. (C) MiR-92a-3p expression is regulated by si-PITPNA-AS1 in SGC7901 cells. D-G: Cell proliferation (D), migration (E), invasion (F), and apoptosis (G) in SGC7901 cells treated with miR-92a-3p mimic and miR-92a-3p inhibitor (AMO- miR-92a-3p). Data are presented as mean ± SEM. Statistic significant differences were indicated: ns, no significance, * P #P

Figure 3. PITPNA-AS1 acts as a sponge for miR-92a-3p in pancreatic carcinoma cells. (A) Subcellular fractionation assay was performed to identify the location of PITPNA-AS1 in SGC7901 cells. (B) The binding sites between PITPNA-AS1 and miR-92a-3p; Luciferase activity of miR-92a-3p mimic with PITPNA-AS1-WT or PITPNA-AS1-MUT. (C) MiR-92a-3p expression is regulated by si-PITPNA-AS1 in SGC7901 cells. D-G: Cell proliferation (D), migration (E), invasion (F), and apoptosis (G) in SGC7901 cells treated with miR-92a-3p mimic and miR-92a-3p inhibitor (AMO- miR-92a-3p). Data are presented as mean ± SEM. Statistic significant differences were indicated: ns, no significance, * P < 0.05 vs. miR-NC, ** P < 0.01 vs. miR-NC; #P <0.05 vs. miR-92a-3p.