Figure 2. Differential gene expression and functional enrichment during FSTL3 overexpression. (A) Volcano map of differentially expressed genes under conditions of elevated FSTL3 levels; adjusted P<0.05. (B) The top 10 differentially expressed genes (linked to FSTL3 overexpression) in gastric cancer disease presented as a gene expression heat map. (C) Gene ontology (GO) term enrichment analysis; adjusted P<0.05. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) biochemical pathway enrichment analysis; adjusted P<0.05. (E) Protein-protein interaction (PPI) network analysis for 122 differentially expressed genes linked to gastric cancer; minimum required interaction score: 0.900. (F) Specific PPI nodes within the full network with MYOCD tool in Cytoscape.