Figure 7. Enrichment and PPI analysis of 56 significantly differentially expressed secretory granule proteins and 140 secreted proteins. (A) The top 10 enriched KEGG pathways of the 56 secretory granules proteins and 140 secreted proteins. (B–C) Candidate proteins interacting with secretogranins in peptide processing in SGs indicated by the PPI network. (D) The expressions of some secretogranins-related candidate neuropeptides and neuroendocrine hormones are verified using real-time PCR. Two-tailed unpaired Student t-tests were performed between the control and treated groups. *Statistically significant with P < 0.05; Error bars are SD; N = 3.