Research Paper Volume 13, Issue 16 pp 20372—20382

Circular RNA circRHOT1 contributes to pathogenesis of non-small cell lung cancer by epigenetically enhancing C-MYC expression through recruiting KAT5


Figure 1. CircRHOT1 is enhanced in clinical NSCLC samples and NSCLC cell lines. (A) The expression of circRHOT1 was analyzed by qPCR in clinical NSCLC tissues (n = 60) and peri-tumor tissues (n = 60). (B) The expression of circRHOT1 was measured by qPCR in the indicated cells. (C) The back-spliced and canonical forms of RHOT1 expression was measured by agarose gel electrophoresis assays and PCR in HCT-116 and HT-29 cells in the presence or absence of RNase R supplementation. (D and E) The localization of circRHOT1 was determined by FISH analysis and 18s and U6 were the controls. mean ± SD, **P < 0.01.