Figure 1. CircRHOT1 is enhanced in clinical NSCLC samples and NSCLC cell lines. (A) The expression of circRHOT1 was analyzed by qPCR in clinical NSCLC tissues (n = 60) and peri-tumor tissues (n = 60). (B) The expression of circRHOT1 was measured by qPCR in the indicated cells. (C) The back-spliced and canonical forms of RHOT1 expression was measured by agarose gel electrophoresis assays and PCR in HCT-116 and HT-29 cells in the presence or absence of RNase R supplementation. (D and E) The localization of circRHOT1 was determined by FISH analysis and 18s and U6 were the controls. mean ± SD, **P < 0.01.