Figure 4. CircRHOT1 epigenetically represses c-MYC expression in NSCLC cells. (A–D) The A549 and H1299 cells were treated with control siRNA or circRHOT1 siRNA. (A) The mRNA expression of c-MYC was tested by qPCR. (B) The protein levels of c-MYC were determined by Western blot analysis. (C) The enrichment of H3K27ac on c-MYC promoter was analyzed by ChIP. (D) The enrichment of RNA polymerase II on c-MYC promoter was analyzed by ChIP. mean ± SD, **P < 0.01.