Research Paper Volume 13, Issue 16 pp 20372—20382

Circular RNA circRHOT1 contributes to pathogenesis of non-small cell lung cancer by epigenetically enhancing C-MYC expression through recruiting KAT5

CircRHOT1 epigenetically regulates c-MYC expression by recruiting KAT5 in NSCLC cells. (A) The interaction of circRHOT1 and KAT5 was measured by RNA pull down in A549 and H1299 cells. (B) The enrichment of KAT5 on c-MYC promoter was analyzed by ChIP. (C) The enrichment of KAT5 on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with circRHOT1 siRNA. (D) The enrichment of H3K27ac on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with KAT5 siRNA. (E) The enrichment of RNA polymerase II on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with KAT5 siRNA. (F) The mRNA expression of c-MYC was tested by qPCR in A549 and H1299 cells treated with KAT5 siRNA. (G) The protein levels of c-MYC were determined by Western blot analysis in A549 and H1299 cells co-treated with circRHOT1 siRNA and KAT5 overexpression vector. mean ± SD, **P

Figure 5. CircRHOT1 epigenetically regulates c-MYC expression by recruiting KAT5 in NSCLC cells. (A) The interaction of circRHOT1 and KAT5 was measured by RNA pull down in A549 and H1299 cells. (B) The enrichment of KAT5 on c-MYC promoter was analyzed by ChIP. (C) The enrichment of KAT5 on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with circRHOT1 siRNA. (D) The enrichment of H3K27ac on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with KAT5 siRNA. (E) The enrichment of RNA polymerase II on c-MYC promoter was analyzed by ChIP in A549 and H1299 cells treated with KAT5 siRNA. (F) The mRNA expression of c-MYC was tested by qPCR in A549 and H1299 cells treated with KAT5 siRNA. (G) The protein levels of c-MYC were determined by Western blot analysis in A549 and H1299 cells co-treated with circRHOT1 siRNA and KAT5 overexpression vector. mean ± SD, **P < 0.01.