Research Paper Volume 13, Issue 17 pp 21202—21215

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma

Expression of UPF1 in EEC cells. (A, B) UPF1 expressed level in four EEC cell lines by qRT-PCR and western blot. (C–F) UPF1 was upregulated or downregulated in Ishikawa and RL952 cells when we overexpressed UPF1(pcDNA3.1-UPF1+ and pcDNA3.1 group) or silenced UPF1(siUPF1 and NC group). siUPF1 has three fragments, we had selected the best effect in silence the expression of UPF1(siUPF1-1) to do the latter experiment.

Figure 2. Expression of UPF1 in EEC cells. (A, B) UPF1 expressed level in four EEC cell lines by qRT-PCR and western blot. (CF) UPF1 was upregulated or downregulated in Ishikawa and RL952 cells when we overexpressed UPF1(pcDNA3.1-UPF1+ and pcDNA3.1 group) or silenced UPF1(siUPF1 and NC group). siUPF1 has three fragments, we had selected the best effect in silence the expression of UPF1(siUPF1-1) to do the latter experiment.