Figure 2. Expression of UPF1 in EEC cells. (A, B) UPF1 expressed level in four EEC cell lines by qRT-PCR and western blot. (C–F) UPF1 was upregulated or downregulated in Ishikawa and RL952 cells when we overexpressed UPF1(pcDNA3.1-UPF1+ and pcDNA3.1 group) or silenced UPF1(siUPF1 and NC group). siUPF1 has three fragments, we had selected the best effect in silence the expression of UPF1(siUPF1-1) to do the latter experiment.