Research Paper Volume 13, Issue 17 pp 21202—21215

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma

mTOR signaling pathway and autophagy related proteins detection. (A) Bioinformatics prediction (<a href="http://www.string-db.org" target="

Figure 5. mTOR signaling pathway and autophagy related proteins detection. (A) Bioinformatics prediction (http://www.string-db.org):mTOR and UPF1 maybe co-expression and interaction. However, mTOR and ULK1 show that both of its relationship have verified. (B, C) Transfected with pcDNA3.1-UPF1+ and pcDNA3.1 or siUPF1 and NC in Ishikawa showed that UPF1 could affect mTOR and downstream’s proteins. (D, E) Overexpressed UPF1 or downregulated UPF1 could inhibit or promote autophagy. LC3I/II and p62 showed autophagy weakened or increased. (FI) The immunofluorescence assays were performed in EEC cells that were transfected with mRFP-GFP-LC3 lentiviral in two different groups. The numbers of GFP and mRFP dots were determined by fluorescent puncta in three high-power fields. The statistical significance between different groups. Data was represented as the mean +/- SEM.*P<0.05, **P<0.01, ***P<0.001.