Figure 2. Effect of CSF on PRLR-JAK-STAT5 pathway in GT1-7. The phosphorylation levels of JAK, STAT5 and the levels of PRLR and kisspeptin in GT1-7 from different groups were detected by western blot assay, and representative bands were shown in (A). The phosphorylation levels of JAK (B) and STAT5 (C), and the level of PRLR (D), and the level of kisspeptin (E) were normalized to control. The results were presented as mean ± SD (n = 3).**p < 0.01 vs. Control group, #p < 0.05, ##p < 0.01 vs. HPRL group.