Figure 3. The upstream components of Fas/FasL-mediated caspase-dependent apoptotic pathway in a normotensive Wistar Kyoto (WKY) group, a spontaneously early aged hypertensive (SHR) group and a hypertension with 12 weeks exercise training (SHR-EX) group. (A) The representative protein levels of TNF α, TNF-R1, Fas receptor (Fas), Fas ligand (FasL), and Fas-associated death domain (FADD), extracted from the cerebral cortices of excised brain in WKY, SHR, and SHR-EX groups were measured by western blot analysis. The α-tubulin was used as an internal control. (B) Bars represent the relative fold changes of protein quantification relative to the control group in TNF α, TNF-R1, Fas, FasL, and FADD on α-tubulin and mean values ± SD (n=6 in each group). *: p<0.05, ***: p<0.001 in comparison between SHR group and WKY rat group. #: p<0.05; ###: p<0.001, in comparison between SHR-EX group and SHR group.