Research Paper Volume 13, Issue 16 pp 20016—20028

Oral administration of berberine represses macrophage activation-associated benign prostatic hyperplasia: a pivotal involvement of the NF-κB

Effects of BB on prostate gland enlargement via androgen-dependent manner in testosterone-induced BPH rat model. The animals were randomly divided into five groups: Con group, BPH group (testosterone-induced BPH rats), Fina group (testosterone-induced BPH rats administered with Fina 5mg/kg), BB 50 group (testosterone-induced BPH rats administered BB 50 mg/kg), and BB 100 group (testosterone-induced BPH rats administered BB 100 mg/kg). (A) Representative photograph of prostate tissues from the testosterone-induced BPH rat model. (B) The prostate weight-to-body weight ratio was estimated. (C) Histopathological analysis was conducted using H&E. Original magnification 100×. (D) Based on histological analysis, the level of TETP was measured and represented. (E) Serum DHT levels were determined using an ELISA kit. (F) The mRNA level of 5α-reductase 2 was quantified using qRT-PCR analysis. ### P

Figure 1. Effects of BB on prostate gland enlargement via androgen-dependent manner in testosterone-induced BPH rat model. The animals were randomly divided into five groups: Con group, BPH group (testosterone-induced BPH rats), Fina group (testosterone-induced BPH rats administered with Fina 5mg/kg), BB 50 group (testosterone-induced BPH rats administered BB 50 mg/kg), and BB 100 group (testosterone-induced BPH rats administered BB 100 mg/kg). (A) Representative photograph of prostate tissues from the testosterone-induced BPH rat model. (B) The prostate weight-to-body weight ratio was estimated. (C) Histopathological analysis was conducted using H&E. Original magnification 100×. (D) Based on histological analysis, the level of TETP was measured and represented. (E) Serum DHT levels were determined using an ELISA kit. (F) The mRNA level of 5α-reductase 2 was quantified using qRT-PCR analysis. ### P < 0.001 versus the Con group; ** P < 0.01, *** P < 0.001 versus the BPH group.