Figure 3. Effect of BB on the expression of NF-κB-mediated inflammatory markers in testosterone-induced BPH rat model. (A) Immunoblotting results showing the protein levels of NOS2 and COX2 in prostatic tissues. Densitometric protein levels are represented as means ± SD, and the plots of each protein are shown. (B) The NOS2 and COX2 mRNA levels in the prostate were quantified by qRT-PCR analysis. Values are represented as means ± SD of data from three separate experiments. (C) Western blot analysis of pIκB-α and IκB-α in prostatic tissue. ### P < 0.001 versus Con group; * P < 0.05, *** P < 0.001 versus the BPH group by ANOVA and Dunnett’s post-hoc test.