Figure 4. Effect of BB on ROS-mediated macrophage activation in testosterone-induced BPH rat model. (A) Western blot analysis of CD68 and NOX4. Data were represented as means ± SD. The mRNA levels of (B) CD68, IL-6 and IL-1β, and (C) CD206, ARG1 and IL-10 were quantified using qRT-PCR. (D) HO-1, NQO1, SOD1 and GPx-1 in prostatic tissues were determined using qRT-PCR analysis. β-actin was used as the housekeeping gene to standardize the gene expression levels. (E) MDA serum concentrations were analyzed using ELISA kit. # P < 0.05, ## P < 0.01, ### P < 0.001 versus Con group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the BPH group by ANOVA and Dunnett’s post-hoc test.