Research Paper Volume 13, Issue 16 pp 20016—20028

Oral administration of berberine represses macrophage activation-associated benign prostatic hyperplasia: a pivotal involvement of the NF-κB

Effect of BB on NF-κB signaling pathway in CM-treated RWPE-1 cell model. (A, B) Prostatic cells stimulated with CM, with or absent 5 and 10 μM BB for 3 days. Western blot analysis of (A) pIκB, IκB and (B) p-p65, p65 in RWPE-1 cell total and nucleus lysates. ### P C, D) IF staining of (C) p-p65 and (D) p65 in CM-treated RWPE-1 cells. (E) The luciferase activity levels were determined in pNF-κB-luc reporter-transfected RWPE-1 cells.

Figure 7. Effect of BB on NF-κB signaling pathway in CM-treated RWPE-1 cell model. (A, B) Prostatic cells stimulated with CM, with or absent 5 and 10 μM BB for 3 days. Western blot analysis of (A) pIκB, IκB and (B) p-p65, p65 in RWPE-1 cell total and nucleus lysates. ### P < 0.001 as compared with the vehicle; *** P < 0.001 as compared with the CM-treated group using ANOVA and Dunnett’s post-hoc test. (C, D) IF staining of (C) p-p65 and (D) p65 in CM-treated RWPE-1 cells. (E) The luciferase activity levels were determined in pNF-κB-luc reporter-transfected RWPE-1 cells.