Research Paper Volume 13, Issue 16 pp 20534—20551

NLRP3 inflammasome activation contributes to the pathogenesis of cardiocytes aging

ROS generation was similar when NLRP3 inflammasomes were inhibited in the cardiocytes aging model. H9c2 cells were pre-treated with or without MCC950 (10μM), a commonly used NLRP3 inhibitor, for 1 hour, and then incubated with or without 10g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that ROS generation was similar no matter NLRP3 inflammasomes were inhibited or not in the cardiocytes aging model. (B) Intracellular ROS was quantified by flow cytometry.

Figure 6. ROS generation was similar when NLRP3 inflammasomes were inhibited in the cardiocytes aging model. H9c2 cells were pre-treated with or without MCC950 (10μM), a commonly used NLRP3 inhibitor, for 1 hour, and then incubated with or without 10g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that ROS generation was similar no matter NLRP3 inflammasomes were inhibited or not in the cardiocytes aging model. (B) Intracellular ROS was quantified by flow cytometry.