Figure 7. Inhibition ROS by NAC attenuated cardiocytes aging induced by D-gal in H9c2 cells. H9c2 cells were pre-treated with or without NAC (1 mM), a commonly used ROS scavenger, for 1 hour and then incubated with or without 10 g/L D-gal for 24 hours. (A) Representative bright-field photomicrographs showed that NAC treatment decreased the percentage of cells expressing β-galactosidase. (B) Flow cytometry analysis was applied to detect the β-galactosidase mean fluorescence intensity after the NAC treatment. (C) The CellEvent™ Senescence Green staining showed that NAC treatment decreased the senescence-associated β-galactosidase expression induced by D-gal. (D) The aging-associated proteins (P53, P21) were detected by western blot, and the corresponding quantification was present.