Research Paper Volume 13, Issue 18 pp 22109—22119

LHX9, a p53-binding protein, inhibits the progression of glioma by suppressing glycolysis

Interaction between LHX 9 and p53. (A) The interaction between LHX9 and fusion protein GST-p53 was detected by GST pull-down assay. 10 μg of GST-p53 fusion protein incubated with SK-N-SH and A172 cell lysis. (B) The interaction between the exogenously expressed Flag-LHX9 and HA-p53 was detected by co-immunoprecipitation assay. (C) The interaction between endogenously expressed LHX9 and p53 was detected by co-immunoprecipitation assay. (D) Schematic diagram of different truncated mutants of p53. (E) The domain of p53 that interacted with LHX9 was identified by co-immunoprecipitation.

Figure 4. Interaction between LHX 9 and p53. (A) The interaction between LHX9 and fusion protein GST-p53 was detected by GST pull-down assay. 10 μg of GST-p53 fusion protein incubated with SK-N-SH and A172 cell lysis. (B) The interaction between the exogenously expressed Flag-LHX9 and HA-p53 was detected by co-immunoprecipitation assay. (C) The interaction between endogenously expressed LHX9 and p53 was detected by co-immunoprecipitation assay. (D) Schematic diagram of different truncated mutants of p53. (E) The domain of p53 that interacted with LHX9 was identified by co-immunoprecipitation.