Research Paper Volume 13, Issue 16 pp 20552—20568

Mediation of circ_RPPH1 on miR-146b-3p/E2F2 pathway to hinder the growth and metastasis of breast carcinoma cells

Carcinogenic role of circ

Figure 2. Carcinogenic role of circ_RPPH1 in BC. (A) qRT-PCR was used to detect the relative expression of circ_RPPH1 in si-circ_RPPH1. (B) qRT-PCR was used to detect the relative expression of circ_RPPH1 in BC cells transfected with si-circ_RPPH1#2. (C) CCK-8 test was used to detect the proliferation of BC cells transfected with si-circ_RPPH1#2. (D, E) Transwell test showed the invasion and migration of BC cells transfected with si-circ_RPPH1#2. Scale bars, 50 μm. (F, G) The changes of cell cycle and apoptosis rate of BC cells transfected with si-circ_RPPH1#2 were detected by flow cytometry. *indicates P < 0.05; **indicates P < 0.01.