Figure 5. circ_RPPH1/miR-146b-3p/E2F2 regulates the growth and metastasis of BC cells. (A) qRT-PCR and WB test were used to detect the relative expression of E2F2 mRNA and protein in co-transfected cells. (B) CCK-8 experiment was used to detect the proliferation changes of cells after co-transfection. (C, D) Transwell test was used to detect the changes of cell invasion number and migration number after co-transfection. Scale bars, 50 μm. (E) The change of apoptosis rate after co-transfection was detected by flow cytometry. *indicates P < 0.05.