Research Paper Volume 13, Issue 17 pp 21232—21250

LncRNA SNHG17 promotes tumor progression and predicts poor survival in human renal cell carcinoma via sponging miR-328-3p

SNHG17 depletion suppressed malignant phenotype of RCC. (A) qRT-PCR assay analysis of the levels of SNHG17 in 786-O and ACHN cell lines transfected with two parallel siSNHG17 (#1 and #2) or siSCR. (B–C) CCK-8 assay (B) and CellTiter-Glo Luminescent cell viability assay (C) analysis of the proliferative ability of RCC cells transfected with the indicated vectors. (D) The activity of caspase-3, -8, and -9 assay analysis of cell apoptosis of RCC cells transfected with the indicated vectors. (E) The wound healing assay analysis of cell migration of RCC cells transfected with the indicated vectors. (F) The transwell assay analysis of cell migration and invasion in RCC cell lines transfected with the indicated vectors. (G) SNHG17 overexpression remarkably increased the volume and weight of tumor xenograft. Scale bar, 1.0 cm. (H) qRT-PCR assay analysis of expression of SNHG17, H2AX and miR-328-3p in the tumor xenograft. (I) Immunohistochemistry staining of Ki-67 in the tumor xenograft. Scale bar, 200 μm. Data are presented as means ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. SCR, scramble control; RCC, renal cell carcinoma. **P ***P

Figure 3. SNHG17 depletion suppressed malignant phenotype of RCC. (A) qRT-PCR assay analysis of the levels of SNHG17 in 786-O and ACHN cell lines transfected with two parallel siSNHG17 (#1 and #2) or siSCR. (BC) CCK-8 assay (B) and CellTiter-Glo Luminescent cell viability assay (C) analysis of the proliferative ability of RCC cells transfected with the indicated vectors. (D) The activity of caspase-3, -8, and -9 assay analysis of cell apoptosis of RCC cells transfected with the indicated vectors. (E) The wound healing assay analysis of cell migration of RCC cells transfected with the indicated vectors. (F) The transwell assay analysis of cell migration and invasion in RCC cell lines transfected with the indicated vectors. (G) SNHG17 overexpression remarkably increased the volume and weight of tumor xenograft. Scale bar, 1.0 cm. (H) qRT-PCR assay analysis of expression of SNHG17, H2AX and miR-328-3p in the tumor xenograft. (I) Immunohistochemistry staining of Ki-67 in the tumor xenograft. Scale bar, 200 μm. Data are presented as means ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. SCR, scramble control; RCC, renal cell carcinoma. **P < 0.01; ***P < 0.001.