Research Paper Volume 13, Issue 17 pp 21232—21250

LncRNA SNHG17 promotes tumor progression and predicts poor survival in human renal cell carcinoma via sponging miR-328-3p

MiR-328-3p is sponged by SNHG17. (A–B) qRT-PCR assay analysis of the expression of miR-328-3p (A) and SNHG17 (B) in 786-O and ACHN cell lines after transfection with the indicated vectors. (C) The potential binding sites of SNHG17 and miR-328-3p were predicted by StarBase v3.0. (D–E) Luciferase activity was analyzed in 786-O and ACHN cells co-transfected with miR-328-3p mimic or NC mimic and SNHG17 wt or mut luciferase reporter vector. (F–G) The level of SNHG17 enriched by Ago2 antibody was detected in 786-O and ACHN cells transfected with miR-328-3p mimic or NC mimic. (H) Spearman’s correlation analysis of the correlation between SNHG17 and miR-328-3p in RCC tissues from the Zhengzhou cohort. Data are presented as means ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. NC, negative control; SCR, scramble; RCC, renal cell carcinoma; ns, not significant. *P **P ***P

Figure 4. MiR-328-3p is sponged by SNHG17. (AB) qRT-PCR assay analysis of the expression of miR-328-3p (A) and SNHG17 (B) in 786-O and ACHN cell lines after transfection with the indicated vectors. (C) The potential binding sites of SNHG17 and miR-328-3p were predicted by StarBase v3.0. (DE) Luciferase activity was analyzed in 786-O and ACHN cells co-transfected with miR-328-3p mimic or NC mimic and SNHG17 wt or mut luciferase reporter vector. (FG) The level of SNHG17 enriched by Ago2 antibody was detected in 786-O and ACHN cells transfected with miR-328-3p mimic or NC mimic. (H) Spearman’s correlation analysis of the correlation between SNHG17 and miR-328-3p in RCC tissues from the Zhengzhou cohort. Data are presented as means ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. NC, negative control; SCR, scramble; RCC, renal cell carcinoma; ns, not significant. *P < 0.05; **P < 0.01; ***P < 0.001.