Research Paper Volume 13, Issue 16 pp 20050—20080

Effects of Siberian fir terpenes extract Abisil on antioxidant activity, autophagy, transcriptome and proteome of human fibroblasts

Changes in mitochondrial respiration and glycolysis level of MRC5-SV40 cells after Abisil treatment. (A) After 16 hours of Abisil treatment, mitochondrial respiration reflected by the level of oxygen consumption rate (OCR) in control (DMSO 0.5%) or Abisil (50 μg/ml) groups (n = 4 per group), following the injection of oligomycin, FCCP and antimycin A/rotenone. (B) the extracellular acidification rate (ECAR) of the medium following injection of glycose, oligomycin and 2 desoxyglucose. (C) The rates of glycolysis level, glycolytic capacity and glycolytic reserve (n = 4 per group). Bars represent mean values with SD, **p p t-test). Glycolytic level – the basal rate of conversion of glucose to pyruvate/lactate; glycolytic capacity – the maximal rate of glycolysis that can be rapidly achieved by a cell; glycolytic reserve – the difference between the glycolytic capacity and the basal glycolytic rate.

Figure 11. Changes in mitochondrial respiration and glycolysis level of MRC5-SV40 cells after Abisil treatment. (A) After 16 hours of Abisil treatment, mitochondrial respiration reflected by the level of oxygen consumption rate (OCR) in control (DMSO 0.5%) or Abisil (50 μg/ml) groups (n = 4 per group), following the injection of oligomycin, FCCP and antimycin A/rotenone. (B) the extracellular acidification rate (ECAR) of the medium following injection of glycose, oligomycin and 2 desoxyglucose. (C) The rates of glycolysis level, glycolytic capacity and glycolytic reserve (n = 4 per group). Bars represent mean values with SD, **p < 0.01, ***p < 0.001 (t-test). Glycolytic level – the basal rate of conversion of glucose to pyruvate/lactate; glycolytic capacity – the maximal rate of glycolysis that can be rapidly achieved by a cell; glycolytic reserve – the difference between the glycolytic capacity and the basal glycolytic rate.