Research Paper Volume 13, Issue 16 pp 20698—20715

m6A regulator-mediated methylation modification patterns and tumor microenvironment infiltration characterization in hepatocellular carcinoma

Landscape of genetic variation of m6A regulators in hepatocellular carcinoma (HCC). (A) Genetic alteration on a query of m6A regulators. (B) The position of the CNV alteration of the m6A regulators on 23 chromosomes from the TCGA-LIHC cohort. (C) The CNV variation frequency of m6A regulators. Red dots represent CNV amplification, while green dots represent CNV deletion. Compared to the other m6A regulators, ZC3H13, YTHDF2 and WTAP had a higher frequency of CNV deletion, while VIRMA, HNRNPC and METTL3 had a higher frequency of CNV amplification. (D) The gene expression levels of 23 m6A regulators in HCC (*, P

Figure 1. Landscape of genetic variation of m6A regulators in hepatocellular carcinoma (HCC). (A) Genetic alteration on a query of m6A regulators. (B) The position of the CNV alteration of the m6A regulators on 23 chromosomes from the TCGA-LIHC cohort. (C) The CNV variation frequency of m6A regulators. Red dots represent CNV amplification, while green dots represent CNV deletion. Compared to the other m6A regulators, ZC3H13, YTHDF2 and WTAP had a higher frequency of CNV deletion, while VIRMA, HNRNPC and METTL3 had a higher frequency of CNV amplification. (D) The gene expression levels of 23 m6A regulators in HCC (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significant). Compared with the normal tissue, the expression of METTL3, METTL14, METTL16, WTAP, VIRMA, RBM15, RBM15B, YTHDC1, YTHDC2, YTHDF1, YTHDF2, YTHDF3, HNRNPC, FMR1, LRPPRC, HNRNPA2B1, RBMX, FTO, and ALKBH5 were upregulated, IGFBP1and IGFBP3 were downregulated in HCC.