Research Paper Volume 13, Issue 16 pp 20738—20747

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway in vivo

Gastric cancer cell MGC-803 promoted angiogenesis in transgenic zebrafish tg (fli1a-EGFP). (A) Biologic morphology of zebrafish. Scale bar: 200 μm. (B) Fluorescence image of subintestinal vessels of the uninjected embryo. Scale bar: 50 μm. (C) Gastric cancer cell lines were injected to the zebrafish embryo, MGC-803 was taken as an example in the figure. Scale bar: 200 μm. (D) MGC-803 cells promoted angiogenesis at 1 dpi compared with control group. The white arrow indicated the tumor cell induced angiogenesis, dpi: days post injection. Scale bar: 50 μm. (E) Quantitative analysis of the length of newly formed vessels in zebrafish with/without inducing by MGC-803.

Figure 2. Gastric cancer cell MGC-803 promoted angiogenesis in transgenic zebrafish tg (fli1a-EGFP). (A) Biologic morphology of zebrafish. Scale bar: 200 μm. (B) Fluorescence image of subintestinal vessels of the uninjected embryo. Scale bar: 50 μm. (C) Gastric cancer cell lines were injected to the zebrafish embryo, MGC-803 was taken as an example in the figure. Scale bar: 200 μm. (D) MGC-803 cells promoted angiogenesis at 1 dpi compared with control group. The white arrow indicated the tumor cell induced angiogenesis, dpi: days post injection. Scale bar: 50 μm. (E) Quantitative analysis of the length of newly formed vessels in zebrafish with/without inducing by MGC-803.