Research Paper Volume 13, Issue 16 pp 20738—20747

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway in vivo

Inhibitory effect of gastric cancer cell line in the zebrafish xenograft model under apatinib treatment. (A) The survival rates and teratogenic rates of zebrafish were detected under different concentrations of apatinib. (B) Fluorescence image of subintestinal vessels in zebrafish induced by MGC-803 with/without apatinib treatment at 1 dpt. (C) Quantitative analysis of the length of newly formed vessels in zebrafish induced by MGC-803 with/without apatinib treatment. Scale bar: 50 μm. (D) Quantitative analysis of the MGC-803 cells proliferation with/without apatinib treatment at 2 dpt. Dpt: days post treatment. (E) Western blot analysis of AKT, phosphorylated AKT (p-AKT), GSK3α, phosphorylated GSK3α (p-GSK3α), GSK3β and phosphorylated GSK3β (p-GSK3β) in injected cells.

Figure 3. Inhibitory effect of gastric cancer cell line in the zebrafish xenograft model under apatinib treatment. (A) The survival rates and teratogenic rates of zebrafish were detected under different concentrations of apatinib. (B) Fluorescence image of subintestinal vessels in zebrafish induced by MGC-803 with/without apatinib treatment at 1 dpt. (C) Quantitative analysis of the length of newly formed vessels in zebrafish induced by MGC-803 with/without apatinib treatment. Scale bar: 50 μm. (D) Quantitative analysis of the MGC-803 cells proliferation with/without apatinib treatment at 2 dpt. Dpt: days post treatment. (E) Western blot analysis of AKT, phosphorylated AKT (p-AKT), GSK3α, phosphorylated GSK3α (p-GSK3α), GSK3β and phosphorylated GSK3β (p-GSK3β) in injected cells.