Research Paper Volume 14, Issue 5 pp 2210—2220

Circular RNA circ_0001006 aggravates cardiac hypertrophy via miR-214-3p/PAK6 axis


Figure 3. circ_0001006 induced hypertrophic in cardiomyocytes. (A) The level of circ_0001006 in cardiomyocytes after transfecting circ_0001006/Vector. (B) Representative immunofluorescence results of cardiomyocytes after transfecting circ_0001006/Vector (The scale bar is 25 μm). (C) Protein/DNA ratio of the cardiomyocytes after transfecting circ_0001006/Vector. (D) The mRNA level of ANP, BNP, and β-MHC in cardiomyocytes. (E) Western blot assay of ANP, BNP, β-MHC protein level in cardiomyocytes. *P < 0.05 and **P < 0.01. Data are shown as mean ± SEM. All experiments were repeated at least three independent experiments.