Research Paper Volume 14, Issue 5 pp 2210—2220

Circular RNA circ_0001006 aggravates cardiac hypertrophy via miR-214-3p/PAK6 axis


Figure 4. Silencing of circ_0001006 prevents cardiomyocyte hypertrophy. (A) The transfection efficiency of sh-circ_0001006 was verified. (B) Representative immunofluorescence results performed the effect of knocking down circ_0001006 (The scale bar is 25 μm). (C) Protein/DNA ratio of the cardiomyocytes after transfecting with sh-circ_0001006/sh-Scr treated with or without Ang II. (D) ANP, BNP, and β-MHC expression level in cardiomyocytes. (E) The expression of β-MHC in cardiomyocytes. *P < 0.05 and **P < 0.01 vs. Control+sh-scr group, #P < 0.05 vs. Control+sh-circRNA. Data are shown as mean ± SEM. All experiments were repeated at least three independent experiments.