Research Paper Volume 14, Issue 5 pp 2210—2220

Circular RNA circ_0001006 aggravates cardiac hypertrophy via miR-214-3p/PAK6 axis


Figure 7. Knockdown of circRNA_0001006 alleviated cardiac hypertrophy in vivo. (A and B) EF (%) and FS (%) were assessed by echocardiography. (C) The histogram results of HW/BW ratios in different groups. (D) Western blot assay analysis of ANP, BNP, and β-MHC expression level in the tissue. (E) Representative histological results of the (H and E) Staining of the mouse heart tissues. (F) The AAV9 transfection efficiency of circ_0001006 in the mouse heart. (G) The miR-214-3p level in heart tissue. (H) The mRNA level of PAK6 in mice. *P < 0.05 and **P < 0.01 vs. Sham+AAV9-sh-scr group, #P < 0.05 vs. Sham+AAV9-sh-circRNA group. Data are shown as mean ± SEM. All experiments were repeated at three independent experiments.