Figure 2. EE alleviates brain damage in CCH rats. (A) TUNEL staining was used to detect neuronal apoptosis in the frontal cortex and hippocampal in each group. Apoptosis-related proteins were detected by Western blot. TUNEL staining. Scale bar = 50 μm. *p < 0.05, vs. Sham group; #p < 0.05, vs. CCH group. N = 5. (B) Western blot. *p < 0.05, vs. Sham group; #p < 0.05, vs. CCH group. N = 3. Sham group, treated with an equal volume of vehicle; CCH group, chronic cerebral hypoperfusion and no treatment; EE group, CCH and treated with EE. *p < 0.05, #p < 0.05.