Research Paper Volume 13, Issue 16 pp 20748—20761

Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats

MK801 blocks the cognitive function of CCH rats in EE. (A) Immunofluorescence staining and Western blot were used to detect the expression of cognitive-related proteins and synaptic plasticity associated proteins. Neurological impairment was measured by the Bederson scoring method. Memory performance was measured in the Morris water maze test. Neuronal dendritic spines changes were detected by Golgi staining. Levels of ActA. (B, C) The content of Ca2+ in the cerebral cortex and hippocampus. Neurological deficit scores. (D–F) Morris water maze test. (G, H) Number and length of dendritic spines. Levels of GAP43, SYN, PSD-95, MAP-2. $p N = 5. *p #p $p

Figure 6. MK801 blocks the cognitive function of CCH rats in EE. (A) Immunofluorescence staining and Western blot were used to detect the expression of cognitive-related proteins and synaptic plasticity associated proteins. Neurological impairment was measured by the Bederson scoring method. Memory performance was measured in the Morris water maze test. Neuronal dendritic spines changes were detected by Golgi staining. Levels of ActA. (B, C) The content of Ca2+ in the cerebral cortex and hippocampus. Neurological deficit scores. (DF) Morris water maze test. (G, H) Number and length of dendritic spines. Levels of GAP43, SYN, PSD-95, MAP-2. $p < 0.05, vs. EE group. EE group, CCH and treated with EE. EEM group, CCH and treated with EE and MK801. N = 5. *p < 0.05, #p < 0.05, $p < 0.05.