Figure 2. PSMC2 knockdown inhibited CCA development in vitro. (A) Cell models with or without PSMC2 knockdown were constructed by transfecting shPSMC2 or shCtrl. The knockdown efficiency of PSMC2 in HCCC-9810 and QBC939 cells was assessed by qPCR and western blotting. (B) MTT assay was employed to show the effects of PSMC2 on cell proliferation of HCCC-9810 and QBC939 cells. (C, D) Flow cytometry was performed to detect cell apoptosis (C) and cell cycle distribution (D) of HCCC-9810 and QBC939 cells with or without PSMC2 knockdown. (E) Human Apoptosis Antibody Array was utilized to analyze the regulatory ability of PSMC2 on expression of apoptosis-related proteins in HCCC-9810 cells. (F) Wound-healing assay was performed to distinguish cell migration of HCCC-9810 and QBC939 cells with or without PSMC2 knockdown. (G) WB was used to detect the expression of EMT related proteins in CCA cell models. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.