Figure 3. The exploration and verification of downstream underlying PSMC2 induced regulation of CCA. (A) A PrimeView Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shPSMC2 and shCtrl groups of HCCC-9810 cells. (B) A PSMC2-induced interaction network was established based on IPA analysis. qPCR (C) and western blotting (D) were used to detect the expression of several selected DEGs in HCCC-9810 cells with or without PSMC2 knockdown. (E) The expression of CDK1 in CCA tissues and normal tissues was evaluated by IHC analysis (scale bar = 50 μm in 200 magnification, scale bar = 20 μm in 400 magnification). (F) The mRNA expression of PSMC2 in CCA cell lines was detected by qPCR. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.