Research Paper Volume 13, Issue 17 pp 21325—21344

Knockdown of PSMC2 contributes to suppression of cholangiocarcinoma development by regulating CDK1

The exploration and verification of downstream underlying PSMC2 induced regulation of CCA. (A) A PrimeView Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shPSMC2 and shCtrl groups of HCCC-9810 cells. (B) A PSMC2-induced interaction network was established based on IPA analysis. qPCR (C) and western blotting (D) were used to detect the expression of several selected DEGs in HCCC-9810 cells with or without PSMC2 knockdown. (E) The expression of CDK1 in CCA tissues and normal tissues was evaluated by IHC analysis (scale bar = 50 μm in 200 magnification, scale bar = 20 μm in 400 magnification). (F) The mRNA expression of PSMC2 in CCA cell lines was detected by qPCR. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P P P

Figure 3. The exploration and verification of downstream underlying PSMC2 induced regulation of CCA. (A) A PrimeView Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shPSMC2 and shCtrl groups of HCCC-9810 cells. (B) A PSMC2-induced interaction network was established based on IPA analysis. qPCR (C) and western blotting (D) were used to detect the expression of several selected DEGs in HCCC-9810 cells with or without PSMC2 knockdown. (E) The expression of CDK1 in CCA tissues and normal tissues was evaluated by IHC analysis (scale bar = 50 μm in 200 magnification, scale bar = 20 μm in 400 magnification). (F) The mRNA expression of PSMC2 in CCA cell lines was detected by qPCR. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.