Figure 4. Knockdown of PSMC2 deepens the effects on CCA cells by CDK1 knockdown. (A, B) The expression of PSMC2 and CDK1 in HCCC-9810 cells transfected with shCtrl, shCDK1 and simultaneous shPSMC2 and shCDK1 were detected by qPCR (A) and western blotting (B). Cell models were subjected to the detection of cell proliferation by Celigo cell counting assay (C), colony formation (D), cell apoptosis (E), cell migration by wound-healing assay (F) and Transwell assay (G). The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.