Figure 5. PSMC2 knockdown inhibited CCA development in vivo. (A) In vivo imaging was performed to evaluate the tumor burden in mice of shPSMC2 and shCtrl groups at day 67 post tumor-inoculation. The bioluminescence intensity was scanned and used as a representation of tumor burden in mice of shPSMC2 and shCtrl groups. (B) 24 days post injection of HCCC-9810 cells with or without PSMC2 knockdown, the volume of tumors formed in mice was measured and calculated at indicated time intervals. (C, D) Mice were sacrificed at day 67 post injection, and the tumors were removed for collecting photos (C) and weighing (D). (E) After removing the tumors, the Ki67 index was evaluated by IHC staining as a representative of tumor growth activity (scale bar = 100 μm in 100 magnification, scale bar = 50 μm in 200 magnification). Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.