Research Paper Volume 13, Issue 18 pp 22120—22133

Metformin inhibits hepatocellular carcinoma development by inducing apoptosis and pyroptosis through regulating FOXO3

Met induced apoptosis and inhibited the migration and the colony formation of HepG2 and Huh7 cells in a dose-dependent manner. (A) Annexin V/PI staining and flow cytometry assays were performed to detect the apoptosis. (B) A transwell assay was used to evaluate cell migration. (C) A colony formation assay was performed to detect the colony formation ability. *P

Figure 2. Met induced apoptosis and inhibited the migration and the colony formation of HepG2 and Huh7 cells in a dose-dependent manner. (A) Annexin V/PI staining and flow cytometry assays were performed to detect the apoptosis. (B) A transwell assay was used to evaluate cell migration. (C) A colony formation assay was performed to detect the colony formation ability. *P<0.05 vs. control group.