Figure 3. Met promoted FOXO3 expression level in HepG2 and Huh7 cells. (A) RNA-seq analysis was performed and the dysregulated genes are shown in the heat map. (B, C) GO/KEGG analysis was carried out to determine clusters of differentially expressed genes with enriched molecular functions. (D–F) Western blots was performed to evaluate protein expressions (G, H) immunofluorescence were performed to evaluate the expression of FOXO3 in HepG2 and Huh7 cell lines after treatment with different concentrations of Met. *P<0.05 vs. control group.