Research Paper Volume 13, Issue 18 pp 22120—22133

Metformin inhibits hepatocellular carcinoma development by inducing apoptosis and pyroptosis through regulating FOXO3

FOXO3 knockdown reversed the effects of Met on HepG2 and Huh7 cell apoptosis, migration, and proliferation. (A, B) qPCR was performed to evaluate the expression of FOXO3 in different groups. (C) Annexin V/PI staining and flow cytometry were performed to detect apoptosis. (D) A transwell assay was used to evaluate the migration. (E) A colony formation assay was performed to detect the colony formation ability. *P

Figure 4. FOXO3 knockdown reversed the effects of Met on HepG2 and Huh7 cell apoptosis, migration, and proliferation. (A, B) qPCR was performed to evaluate the expression of FOXO3 in different groups. (C) Annexin V/PI staining and flow cytometry were performed to detect apoptosis. (D) A transwell assay was used to evaluate the migration. (E) A colony formation assay was performed to detect the colony formation ability. *P<0.05, Met vs. control group, Met + siFOXO3 vs. Met + si-nc group.