Research Paper Volume 13, Issue 18 pp 22120—22133

Metformin inhibits hepatocellular carcinoma development by inducing apoptosis and pyroptosis through regulating FOXO3

Met induced pyroptosis in HepG2 and Huh7 cells. (A) Morphologic observation of the HepG2 and Huh7 cell lines after treatment with different concentrations of Met. (B, C) Western blot was performed to evaluate the expression level of pyroptosis-related proteins NLRP3, cleaved-caspase1, IL-1β, IL-18, and GSDMD after Met treatment. (D, E) Western blot was performed to evaluate the expression level of pyroptosis-related proteins NLRP3, cleaved-caspase1, IL-1β, IL-18, and GSDMD after co-treatment with different concentrations of Met and si-FOXO3. (F) MTT was used to evaluate the cell viability of HepG2 and Huh7 cells. *P#P

Figure 6. Met induced pyroptosis in HepG2 and Huh7 cells. (A) Morphologic observation of the HepG2 and Huh7 cell lines after treatment with different concentrations of Met. (B, C) Western blot was performed to evaluate the expression level of pyroptosis-related proteins NLRP3, cleaved-caspase1, IL-1β, IL-18, and GSDMD after Met treatment. (D, E) Western blot was performed to evaluate the expression level of pyroptosis-related proteins NLRP3, cleaved-caspase1, IL-1β, IL-18, and GSDMD after co-treatment with different concentrations of Met and si-FOXO3. (F) MTT was used to evaluate the cell viability of HepG2 and Huh7 cells. *P<0.05 vs control group, #P<0.05 vs Met+vehicle group.