Research Paper Volume 13, Issue 16 pp 20774—20792

NF-κB inducible miR-30b-5p aggravates joint pain and loss of articular cartilage via targeting SIRT1-FoxO3a-mediated NLRP3 inflammasome

Inhibition of NF-κB reduced miR-30b-5p levels and IL-1β-mediated HC-A cell injury. BAY 11-7082 was added to IL-1β-mediated HC-A cells and miR-30b-5p mimics were given. (A, B) NF-κB and miR-30b-5p expression was monitored by WB and RT-qPCR, respectively. (C, D) Cell proliferation and apoptosis were determined by CCK8 and flow cytometry, respectively. (E) Expression of Bax and Cleaved-Caspase3 was determined by WB. (F, G) RT-qPCR and WB were conducted to compare the levels of IL-1β, TNF-α, MMP3 and MMP13. (H, I) The expression of SIRT1 and FoxO3a was assessed by RT-qPCR. (J) WB detected the expression of NLRP3-ASC-cleaved Caspase1 inflammasomes. **PP

Figure 9. Inhibition of NF-κB reduced miR-30b-5p levels and IL-1β-mediated HC-A cell injury. BAY 11-7082 was added to IL-1β-mediated HC-A cells and miR-30b-5p mimics were given. (A, B) NF-κB and miR-30b-5p expression was monitored by WB and RT-qPCR, respectively. (C, D) Cell proliferation and apoptosis were determined by CCK8 and flow cytometry, respectively. (E) Expression of Bax and Cleaved-Caspase3 was determined by WB. (F, G) RT-qPCR and WB were conducted to compare the levels of IL-1β, TNF-α, MMP3 and MMP13. (H, I) The expression of SIRT1 and FoxO3a was assessed by RT-qPCR. (J) WB detected the expression of NLRP3-ASC-cleaved Caspase1 inflammasomes. **P<0.01, ***P<0.001. N=3.